This collection documents Hunt's personal and professional life and includes resumes and articles about Hunt; diaries and address books; college and graduate school papers (including a draft of Hunt's docotral thesis), notebooks, and transcripts; speeches and conference materials; book reviews, articles, and manuscript drafts; syllabi for Hunt's classes and for classes by others; reports, speeches, and questionnaires related to Hunt's volunteer work with Transition House (battered women's shelter) and her work in Juneau, Alaska, for the Women in Transition Program; correspondence re: Radcliffe women's groups (including the \"Radcliffe Lesbians\" group) and Radcliffe Union of Students; musical compositions by Hunt; and printed material re: issues of interest to her (politics, gay rights, women's rights, debates about pornography, etc.) The collection also includes correspondence (with friends, lovers, family, and colleagues) documenting Hunt's personal and emotional life; some folders are closed to protect the privacy of individuals with whom she was involved and other individuals discussing personal details of their lives. Relatively little material related to Hunt's work at Amherst College is included; some of that material is, however, located at the Amherst College Library Archives & Special Collections. Most folder titles were created by the archivist; Hunt's folder titles, when used, appear in quotation marks.Electronic records were received on 117 3.5\" disks, 13 zip disks, 11 compact discs, and 1 USB memory stick. Disks were imaged using FTK Imager and selected documents were converted to PDF/A for preservation and delivery. E-mail (#E.5 and #E.6) was extracted using ePADD, a software package developed by Stanford University. #E.5 is closed until January 1, 2064. An appointment is necessary to access #E.6. Please contact the Reference Desk.Series I, BIOGRAPHICAL AND PERSONAL, 1961-2013 (#1.1-15.5, E.1-E.3), articles about Hunt and various versions of her resume; address and appointment books; passports and Hunt's will; programs for musical performances; and diaries. The series also includes transcripts and applications (funded and otherwise) for grants and fellowships. The bulk of the series consists of papers (some with instructor's comments) and notebooks from Hunt's studies at the University of Pennsylvania, Radcliffe College, Harvard Divinity School, and New York University, with some earlier schoolwork also included, as well as a notebook with messages from her classmates at the Internationale Quakerschool in the Netherlands. Of particular note is the material related to Hunt's work with the Radcliffe-Harvard's Women's Center, the Radcliffe Union of Students, and the effort to establish a women's studies program at Harvard. Also of note is the material related to Hunt's discrimination suit against Harvard Book Store, a Dutch button with the slogan \"We Women Demand Legalized Abortion,\" and a \"learning agreement\" composed in 1976 in which Hunt reflects on her plans for the immediate academic year and beyond, noting \"If I have a 'calling' (to return to the root meaning of the word) it is to participate in some way in the process of liberation....I'm primarily interested in being an effective activist whatever it takes.\" (#2.9). The series is arranged alphabetically.Series II, CORRESPONDENCE, 1959-2013 (#15.6-35.1, E.4-E.16), includes correspondence with family members, including Hunt's parents and siblings, her maternal grandparents, her great aunt Louise Fox Connell, and her aunt Virginia Hunt Wedgwood. Hunt's correspondence with her parents includes descriptions of their work and travels and also touches on her conflicted feelings towards them. Of note are a letter in which a teenaged Hunt shares her thoughts on drugs, sex, and other lifestyle choices (#20.18) and a letter from Hunt's sister Kitty analyzing Edward R. Hunt's personality (#20.17). Other correspondents include professional colleagues (including Mary Beth Norton, Anna Davin, Jan Lambertz, and many other women active in the areas of history and women's studies), friends, and romantic partners, with additional correspondence related to historical associations and feminist, political, and gay rights organizations with which Hunt was involved. Topics include conference planning and other academic and literary projects, political activism, details of daily life, travel, and relationship issues, such as personality conflicts, differing goals and expectations, and problems caused by long distance relationships. Letters of recommendation for students and colleagues are also included. The series is arranged with alphabetical correspondence first, followed by chronological correspondence. Some correspondents are identified by initial, rather than by name, to protect their privacy.Series III, PROFESSIONAL,1946-2012, (#35.2-49.3, E.17-E.20), includes correspondence, reports, and grant applications related to Hunt's work with the American Friends Service Committee, Transition House, the Women in Transition Program at the University of Alaska, and the Displaced Homemakers Program at Bronx Community College. The Women in Transition material also includes questionnaires completed by women in the program, with questions such as \"What are some things you could do if you had been turned down for a scholarship Assume you couldn't go to school without financial help.\"The series also includes notes, programs, speeches, and planning materials for conferences in which Hunt participated. Conferences Hunt regularly attended include the Berkshire Conference on the History of Women, conferences of the American Society for Eighteenth-Century Studies, the North American Conference on British Studies, and the Northeast Conference on British Studies, as well as conferences focused on the lives and roles of women in the early modern era and on lesbian and gay studies. Speeches by Hunt include \"Women, Credit and the Fiscal-Military State in Late 17th and Early 18th Century London,\" \"Women's History and the Digital Future,\" and \"Gender and Islamic Law in Global Historical Perspective.\" Syllabi Hunt collected on topics such as women's studies and history and lesbian/gay studies are also included here, as well as printed material on women's rights and gender violence. Also included are book reviews and reader reports by Hunt; correspondence with publishers; and articles and book chapters. Material related to Hunt's book, The Middling Sort: Commerce, Gender and the Family in England 1660-1780, includes a contract, publicity, and a promotional questionnaire completed by Hunt; readers' reports, with Hunt's reactions; reviews; and correspondence related to prizes the book received. The series also includes entries Hunt wrote for the Oxford Dictionary of National Biography and correspondence, draft articles, and reader reports for issues of Radical History Review co-edited by Hunt. Also of note is the material on the Lesbian Herstory Archives (Brooklyn, New York), and an interview Hunt conducted with the one of the Archives' founders, Joan Nestle, later published in the Boston-based newspaper Gay Community News. The series is arranged alphabetically.Series IV, PHOTOGRAPHS, AUDIOVISUAL, AND OVERSIZED, 1956-2001 (#PD.1-PD.3, Vt-262.1, FD.1-FD.2, F+D.1-F+D.2, OD.1, SD.1), is arranged by format. The series includes photographs of Hunt alone and with others, including fellow recipients of honorary degrees at Uppsala University; flyers promoting the services of the Radcliffe-Harvard Women's Center; and oversized items removed from elsewhere in the collection. The series also includes a videotape of four women recounting their experiences with illegal abortion, recorded at the Reproductive Rights Conference held at Hampshire College in 1989.Most of the photographs in this collection are or will be digitized and available online.
Technical sensitivity, reproducibility, and efficiency of our assay. (A) SARS-CoV-2 RNA control was diluted in several fold dilution steps (105, 104, 103, and 102 copies) and measured with RT-qPCR in four or eight replicates (different symbols characterize the different replicates) using the virus specific N1 (blue) and N2 (orange) primer/probe sets. Cq values were plotted against viral synthetic RNA copies. (B) SARS-CoV-2 RNA control was added to a pool of negative patient samples to have 106, 105, 104, 103, and 102 RNA copies/reaction before the RNA extraction step followed by RT-qPCR. Cq values for N1 (light blue, including RNA extraction) and N2 (red, including RNA extraction) were monitored and plotted against the increasing number of RNA copies used. The Cq values are compared with the measurements of RNA copies that have been measured without including an RNA extraction step (see plot (A); N1, blue; N2, orange). The dotted line visualizes the difference in Cq values of detecting the same number of RNA molecules (e.g., 104 copies) that are either present before (N1 and N2, no extraction) or after (N1 and N2, extraction) RNA extraction. (C) Two positive patient samples collected in COBAS lysis buffer were diluted in a series of 1:5 (1:1, 1:5, 1:25, 1:125, 1:625) using buffer 6 before extracting viral RNA followed by RT-qPCR. Detected Cq values for N1 (square) and N2 (asterisk) of both samples (sample 1, blue; sample 2, orange) were plotted against the dilution factor in a log scale. For all plots, the linear correlation (R2) was higher than 0.98.
Effect of lysis buffer on test performance. Shown are measured Cq values of a positive sample pool diluted in different lysis buffers in a 1:10 dilution series. Different colored bars in the plot represent the buffers used measured with our in-house system (JKU system) and the Roche COBAS system. 59ce067264